In this report, deletions of key genes required for the biogenesis of flagella and pili led to the generation of M. maripaludis strains lacking pili or flagella or both appendages.

Mutants missing either or both flagella and pili http://www.selleckchem.com/products/PLX-4032.html were shown to be extremely compromised in their ability to attach to any of the many potential substrates tested compared with wild-type cells. These studies show that besides their previously documented role in swimming (Chaban et al., 2007), flagella of M. maripaludis are also critical for attachment and are involved in cell-to-cell contacts. Similarly, a role in attachment is demonstrated for pili, the first role assigned to these unusual organelles, in this organism. Very few studies on any archaea have been devoted to determining the functions of the several different types of archaeal appendages. Some organisms studied have only pili or flagella and some lack appropriate genetic systems in which to further characterize the roles of the various appendages. In Methanothermobacter thermautotrophicus, pili are the sole known surface appendages. Cells grown planktonically are poorly piliated; the expression of surface

pili is much enhanced, however, under conditions where the cells adhere (Thoma et al., 2008). These pili were shown to be essential for the adherence of cells to a variety of surfaces, as antibodies to the major pilus structural protein lead to detachment of the cells. A genetic system that would allow the generation of nonpiliated mutants in this species is not available. This Regorafenib ic50 study was the first to demonstrate a role for pili in any archaeon. In the hyperthermophile, Pyrococcus furiosus, on Phospholipase D1 the other hand, only flagella have been reported on the cell surface and these organelles were shown to be responsible for the adherence of cells to many types of surfaces, including ones found in the organism’s natural environment (Nather et al., 2006), although adhesion to glass and mica was limited, as observed here with M. maripaludis. Again, lacking a genetic

system in which to generate nonflagellated mutants, it was shown that adherent cells could be detached by antibodies directed against flagella. This was the first report of an adhesion role for archaeal flagella. In some of the electron micrographs, large cables of flagella can be observed to leave the cell before unwinding to the single flagella that are involved in adherence, as observed for M. maripaludis. Large cables of flagella were also seen to connect cells, an additional novel role for archaea flagella and an observation again made in this study for the flagella of M. maripaludis. Pyrococcus furiosus can also attach to Methanopyrus kandleri cells via its flagella, forming a unique archaeal bispecies biofilm (Schopf et al., 2008). Cable-like groups of flagella were shown to mediate cell-to-cell contact and attachment to gold grids in Methanocaldococcus villosus (Bellack et al., 2010).