The results demonstrated that treatment with GL inhibited Akt phosphorylation and ERK JNK PI3K somewhat, showing GLs capability to control CD4 T cells via PI3K/, ERK and JNK AKT signaling pathways. The outcome also indicated that ERK, JNK and PI3K/Akt paths will be the potential targets for inhibiting liver fibrosis progression. Similarly, phosphorylation of ERK, JNK and AKT was induced by ConA, inhibitors and hdac3 inhibitor of ERK, JNK and PI3K/AKT dramatically reduced the HBcAg induced PD 1 upregulation on CD4 T cells. To summarize, GL alleviated ConA induced irritation and fibrosis progression in livers of mouse models via the inhibition of CD4 T cell growth in response to ConA via ERK, JNK and PI3K/ AKT pathway. The authors assert that they have no competing interests. People of the Bcl 2 family connect to a number of proteins and consequently increase the rupture of the outer membrane or the Skin infection mitochondria, leading to a release of pro apoptotic proteins and the triggering of apoptosis. Since a failure of the inactivation of professional apoptotic pathways, or the service of anti apoptotic pathways, might occur in the complex regulation process, a disregulation of the Bcl 2 family proteins might lead to the growth of cancer. The growth of inhibitors against Bcl 2 or Bcl XL for the use as anti cancer drugs may be promising, as there is a real chance to overcome the characteristics of these proteins. Using our internally database with over four million materials, a virtual screening based on 2D and 3D characteristics is conducted. Being known components, BH3I 2 and BH3I 1 might be used as lead compounds. The database enables online screenings for small molecules with similar structures or similar chemical properties. Chemical characteristics of substances are compared through the use of fingerprints, to find out, whether a 2D similarity can be acquired. If calculated fingerprints are available, Ubiquitin ligase inhibitor they could be used to determine the Tanimoto coefficient, which describes chemical similarities between two molecules. In general, a Tanimoto coefficient above 0. 85 makes an informed guess, that the examined substances have similar properties. Chemical similarity isn’t necessarily associated with a similarity in organic characteristics. By firm body architectural position, two elements and conformers thereof, could be compared regarding their 3D structure. For this function, the superposition formula is used, that was developed within our group. The Lipinski Rule offive is used, to be able to produce a record on the bioavailability of a substance, which might be used as a medicine. Substances that do not obtain the Rule of five shouldn’t be viewed as candidates for a drug. Promising candidates were docked in Bcl XL using the system GOLD, which uses a genetic algorithm to examine the full range of ligand conformational flexibility with partial flexibility of the protein. It mimics the procedure of evolution by applying genetic operators to an assortment of putative poses to some single ligand.