The results obtained with the procedure always coincided with those from the GDC-0941 ic50 Standard techniques from the clinical laboratory. The concentration where the presence

of the background of DNA fragments was observed coincided with that where nucleoids were released, in gram-negative strains. Nevertheless, in spite of releasing of nucleoids, the background of DNA fragments was very scarce or undetectable in susceptible gram-positive strains at the doses employed (Table 1 Figure 9). Table 1 Microorganisms evaluated for susceptibility-resistance to antibiotics that inhibit peptidoglycan synthesis Gram Bacteria Antibiotics- CLSI MIC Interpretative Standard (μg/mL) CLSI Category I-BET-762 MIC (μg/ml) Drug concentration at which the Cytoskeletal Signaling inhibitor nucleoids were spread – and DNA fragments were released Gram – Acinetobacter baumannii Imipenem: ≤ 4 – 8 – ≥16 (SI, R) Susceptible 2 4-4 Gram – Acinetobacter baumannii Imipenem: ≤ 4 – 8 – ≥16 (SI, R) Intermediate 8 16-16 Gram – Acinetobacter baumannii Imipenem: ≤ 4 – 8 – ≥16 (SI, R) Resistant > 16 No nucleoids-No fragments Gram – Acinetobacter baumannii Imipenem: ≤ 4 – 8 – ≥16 (SI, R) Resistant > 16 No nucleoids-No fragments Gram – Acinetobacter baumannii Ceftazidime: ≤ 8 – 16 – ≥32 (S, I, R) Susceptible 4 8-8 Gram – Acinetobacter baumannii Ceftazidime: ≤ 8 – 16 – ≥32 (S, I, R) Intermediate 12 32-32

Gram – Acinetobacter baumannii Ceftazidime: ≤ 8 – 16 – ≥32 (S, I, R) Resistant

> 256 No nucleoids-No fragments Gram – Enterobacter cloacae Imipenem: ≤ 1 – 2 – ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Enterobacter cloacae Imipenem: ≤ 1 - 2 - ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Enterobacter cloacae Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R) Susceptible < 1 4-4 Gram - Enterobacter cloacae Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R) Susceptible < 1 4-4 Gram - Escherichia coli Ampicillin: ≤ 8 - 16- ≥32 (S, I, R) Susceptible 2 8-8 Gram - Escherichia coli Ampicillin: ≤ 8 - 16- ≥32 (S, I, R) Intermediate 12 16-16 Gram - Escherichia coli Ampicillin: ≤ 8 - 16- ≥32 (S, I, R) Resistant 256 No nucleoids-No fragments Gram - Escherichia Alectinib coli Ceftazidime: ≤ 4 -8- ≥16 (S, I, R) Susceptible 0.25 4-4 Gram – Escherichia coli Ceftazidime: ≤ 4 -8- ≥16 (S, I, R) Resistant 32 No nucleoids-No fragments Gram – Klebsiella oxytoca Imipenem: ≤ 1 – 2 – ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Klebsiella oxytoca Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R) Susceptible < 1 4-4 Gram - Klebsiella spp. Imipenem: ≤ 1 - 2 - ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Klebsiella spp. Imipenem: ≤ 1 - 2 - ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Klebsiella spp. Imipenem: ≤ 1 - 2 - ≥4 (S, I, R) Susceptible < 1 1-1 Gram - Klebsiella spp. Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R) Intermediate 8 16-16 Gram - Klebsiella spp. Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R) Resistant > 16 No nucleoids-No fragments Gram – Klebsiella spp.