Aerial hyphae

common, several mm long and high, often branched in right angles. Autolytic activity inconspicuous, more pronounced at 15°C, coilings frequent. No chlamydospores, only some hyphal thickenings seen. No diffusing pigment, no distinct odour noted. Conidiation noted after (8–)13 days at 25°C, developing slowly, examined after 24–52 days; first scant on distal aerial hyphae and in short shrubs close to the distal margin. Shrubs growing to white fluffy tufts appearing in a broad distal zone, spreading back across the entire plate. Tufts compacting selleck compound to pustules 0.5–3 mm diam, to 1 mm thick, with roundish or irregular outline, often semiglobose or oblong, remaining transparent; of a loose PCI-34051 cell line reticulum with branches conspicuously at right angles and straight main axes emerging from the reticulum in right angles. Main axes 100–150(–200) μm long, first appearing as erect sterile elongations. Branches and elongations beset with numerous small drops, appearing verrucose under low magnification, but dissolving in microscopic mounts. Elongations becoming fertile, i.e. conidiation first terminal, concentrated in the pustule periphery, later also within tufts, dense, eventually making them opaque. Conidiophores (main axes and side branches) 4–6 μm wide

basally, attenuated upwards to 2.5–4 μm, smooth in microscopic preparations, sometimes with clamp-like thickenings, stipitate, with branches generally widely spaced, typically with a short terminal cluster of phialides GSK2118436 in vivo and/or few short perpendicular branches, and with or without paired or unpaired, short, 1–4 celled side branches along their length. Clusters and side branches 15–40(–60)

μm long, generally in right angles, typically of a terminal phialide or whorl of phialides and additional phialides or short, sometimes rebranching, branches on 1–3 levels below the terminal whorl, each branch with a whorl of phialides. Conidiophores with a regular tree-like shape uncommon. Phialides solitary or divergent, sometimes parallel, in whorls of 2–5(–6), often PRKD3 supported by short cells 4–6 × 2.5–4 μm. Conidia formed in small numbers in minute wet heads to 20 μm diam, often densely packed. Phialides (4.5–)5.5–9.0(–12.5) × (2.3–)2.5–3.2(–3.5) μm, l/w (1.5–)1.9–3.1(–4.7), (1.4–)1.8–2.5(–3.0) μm wide at the base (n = 78), lageniform, less commonly ampulliform, mostly inaequilateral, straight or curved, rarely sinuous, with widening in variable position, mostly in or below the middle. Conidia (2.5–)2.8–3.5(–4.2) × 2.0–2.5(–3.0) μm, l/w (1.0–)1.2–1.5(–1.8) (n = 125), hyaline, ellipsoidal, less commonly subglobose or oblong, smooth, with several minute guttules, scar indistinct. At 15°C no conidiation seen. Habitat: on recently dead culms of Juncus effusus, and gramineous and herbaceous plants. Distribution: Europe (Denmark, Germany, United Kingdom).