After washing with PBS, the sections were covered

with EnVision plus (Dako) for 40 min at 37°C and washed in PBS. Antigenic sites bound by the antibody were identified by reacting the sections with a mixture of 0.05% 3,3′-diaminobenzidine tetrahydrochloride in 50 mM Tris-HCl buffer and 0.01% hydrogen peroxide. Sections were counterstained with methyl green. Acknowledgements The authors thank T. Hirayama for providing H. pylori strain (ATCC 49503); J. Fujisawa for providing reporter plasmid κB-LUC; and D. R. Alessi for providing the dominant negative mutant of Akt. This work was supported in part by the Takeda Science Foundation and Grants-in-Aid for Scientific Research on Priority Areas from Ministry of Education, Culture, Sports, Science and Technology (20012044) and Scientific Research (C) from Japan Society for the Promotion of Science (19591123). selleck inhibitor References 1. Hocker M, Hohenberger P:Helicobacter pylori virulence factors-one part of a big picture. Lancet 2003, 362:1231–1233.CrossRefPubMed 2. Houghton J, Wang TC:Helicobacter pylori and gastric cancer: a new paradigm for inflammation-associated epithelial cancers. Gastroenterology 2005, 128:1567–1578.CrossRefPubMed 3. Kwok T, Zabler D, Urman S, Rohde M, Hartig R, Wessler S, Misselwitz R, Berger J, Sewald N, König W, Backert S:Helicobacter exploits click here integrin for type IV secretion and kinase activation. Nature 2007, 449:862–866.CrossRefPubMed 4. Moss SF, Sood S:Helicobacter pylori. Curr

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