Data expressed as mean ± SD or a representative of one of three similar experiments unless otherwise indicated. Comparisons were made between control and treated groups or the

entire intra group using one way and two ways ANOVA with post Bonferroni GSK269962 mouse test through GraphPad Prism 5.00.288 statistical analysis software by GraphPad Software, Inc. p -values * < 0.01 were considered significant when compared to untreated control or respective DQQ treated cells. Cells treated with different doses of DQQ for different time frames, displayed inhibited viability in a dose and time dependent manner (Fig. 1B, C). The IC50 of DQQ against K562 and MOLT-4 was determined at different time points which come out to be 24 μM, 19 μM, 7 μM and 4 μM in 6 h, 12 h, 24 h and 48 h, respectively in MOLT-4 cells (Fig. 1B, C), while in case of K562 cells the IC50 values were 62 μM, 36 μM, 16 μM and

12 μM in 6 h, 12 h, 24 h and 48 h, respectively (Fig. 1B, C). The IC50 values of DQQ in K562 cells were comparatively higher than observed in MOLT-4 cells. Thus, the MOLT-4 cell line was taken for further mechanistic studies. Apoptosis was one of the modes of leukemic cell death induced by DQQ, which was further confirmed by a battery of apoptosis assays Hoechst and annexin-V staining, cell cycle and mitochondrial potential analysis. Phase contrast and nuclear microscopy results revealed that DQQ substantially induced apoptosis in MOLT-4 cells in a dose dependent manner (Fig. 2A, B). Nuclei of untreated MOLT-4 cells appeared round in shape, while treatment with DQQ resulted in nuclear condensation NVP-BGJ398 order and the formation of apoptotic bodies. The morphological changes were accompanied by an increase in the number of scattered apoptotic bodies, indicated by white arrows (Fig. 2B). AnnexinV/PI staining is widely used to distinguish between apoptosis and necrotic population.

The results of AnnexinV/PI staining suggested that the cell death induced by DQQ was of apoptotic nature as the amount of population positive for PI was negligible. The percentage of apoptotic population was significantly higher (10-20 times) in DQQ treated MOLT-4 cells as compared to untreated control (Fig. 2 C). Apoptosis was further confirmed by cell cycle analysis using propidium iodide staining. Measurement of DNA content Venetoclax cell line makes it possible to identify apoptotic cells and cell cycle phase specificity. The results revealed that DQQ substantially induced 3-10 times increase in hypo-diploid sub-G0 DNA fraction (apoptotic, <2nDNA) in cell cycle phase distribution (Fig. 2D). The sub-G0 fraction (apoptotic) was 7% in control cells, which increased up to 69% after 10 μM concentrations of DQQ treatment in MOLT-4 cells (Fig. 2D). The early event which was associated with DQQ induced apoptosis was found to be loss of mitochondrial membrane potential (Δψm). Mitochondrial membrane potential loss is one of the important and commonly occurring events in apoptosis.