Expression of PEDF and CD34-positive vessels (microvessel density, MVD) in tumor samples was examined by immunohistochemistry. The relationship between PEDF expression and clinicopathologic features including tumor diameter, MVD, and survival was evaluated by univariate and multivariate analyses. Results: Of 203

patients, 116 cases (57.1%) were positive for PEDF. A significant association was found between PEDF expression and high MVD (p = 0.012). PEDF expression was inversely correlated with grade (p = 0.004) and pT stage (p = 0.001). Multivariate analysis using the Cox regression model indicated CBL0137 that PEDF expression was an independent favorable prognostic factor (risk ratio, 4.105; p = 0.001). However, no significant correlation was found between PEDF expression and age, gender, IWR-1-endo clinical trial tumor diameter or lymph node invasion. Conclusion: Our findings suggest that PEDF may be a favorable prognostic indicator in patients with CCRCC. Copyright (C) 2010 S. Karger AG, Basel”
“Mechanistic

inter-relationships in sinks between sucrose compartmentation/metabolism and phloem unloading/translocation are poorly understood. Developing grain legume seeds provide tractable experimental systems to explore this question. Metabolic demand by cotyledons is communicated to phloem unloading and ultimately import by sucrose withdrawal from the seed apoplasmic space via a turgor-homeostat mechanism. What is unknown is how metabolic demand is communicated to cotyledon sucrose transporters responsible for withdrawing check details sucrose from the apoplasmic space. This question was explored here using a pea rugosus mutant (rrRbRb) compromised in starch biosynthesis compared with its wild-type counterpart (RRRbRb). Sucrose influx into cotyledons was found to account for 90% of developmental variations in their absolute growth and hence starch biosynthetic rates. Furthermore, rr and RR cotyledons shared identical response

surfaces, indicating that control of transporter activity was likely to be similar for both lines. In this context, sucrose influx was correlated positively with expression of a sucrose/H(+) symporter (PsSUT1) and negatively with two sucrose facilitators (PsSUF1 and PsSUF4). Sucrose influx exhibited a negative curvilinear relationship with cotyledon concentrations of sucrose and hexoses. In contrast, the impact of intracellular sugars on transporter expression was transporter dependent, with expression of PsSUT1 inhibited, PsSUF1 unaffected, and PsSUF4 enhanced by sugars. Sugar supply to, and sugar concentrations of, RR cotyledons were manipulated using in vitro pod and cotyledon culture.