Formulation Calcitriol supplier analysis The percentage Assay of LORN and PCM in two different tablet samples Lornasafe-plus and Lorsaid-P were calculated and recorded in Table 4. Table 4 Summary of validation Specificity The specificity of the method was ascertained by the absence of any other peak of placebo [Figure 2].The validation summary is given in Table 4. Figure 2 Chromatogram of constituted tablet placebo CONCLUSION The developed HPTLC method was found to be simple, specific, precise, accurate, and reproducible and can be used for the routine estimation of LORN and PCM in the combined tablets dosage form available in market. The developed method is found to be less sensitive but more accurate and specific than the previously published method. ACKNOWLEDGMENT Authors are thankful to Cirex pharmaceutical Ltd (Gundla, Mandal district, A.

P., India) and Cadila Pharmaceutical Ltd (Dholka, Ahmedabad, Gujarat, India) for providing the gift sample of LORN and PCM standard. Footnotes Source of Support: Nil Conflict of Interest: None declared.
Capsaicin (CAP) is the main alkaloid responsible for pungency in chillies and is used as a counterirritant balm for external application and it is also used in creamy to provide external pain relief for arthritis patients.[1] To date, research has shown that capsaicinoids, and CAP in particular, have a wide variety of biological and physiological activities which provide them functions, such as antioxidants, anticarcinogenics, promotion of energy metabolism and suppression of fat accumulation, and inflammations.

However, the potential applications of these molecules are limited by the irritation caused by their pungency.[2] Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is a crystalline, lipophilic, colorless, and odorless alkaloid Cilengitide with the molecular formula C18H27NO3 [Figure 1]. Its molecular weight is 305.40 g/mol, and it is fat-, alcohol- and oil-soluble. CAP displays cis/trans isomerism because the double bond prevents internal rotation. CAP is always found as the trans isomer because in the cis form, the �CCH(CH3)2 and the longer chain on the other side of the double bond will be close together, causing them to repel each other slightly. This additional strain imposed causes the cis isomer to be a less stable arrangement than the trans isomer.[2] Figure 1 Chemical structure of capsaicin CAP is known to be effectively absorbed topically from the skin. In a study of 12 subjects topically administered, a 3% CAP solution in three different vehicles (70% isopropyl alcohol; mineral oil; and propylene glycol in 20% alcohol), CAP was shown to be rapidly absorbed and to quickly reach maximum concentration when CAP is applied topically. The half-life of CAP was approximately 24 h.[3].