Milk from goats heterozygous for the deletion contained a high content of (alpha(s1)-CN compared with the milk from goats homozygous for the deletion that

contained very low amounts of alpha(s1)-CN. Milk from heterozygous goats contained the highest fat and protein content and exhibited the best coagulation properties; therefore, it was more preferable for cheese making. Cheese manufactured from milk from the heterozygous goats obtained a better and more stable cheese quality than did cheese made from the homozygous goats. The latter cheese had a higher moisture content, a more often rancid flavour and a different composition of free amino acids. (C) 2014 Elsevier B.V. All rights reserved.”
“The participation of opioids in the antinociceptive effect of electroacupuncture was evaluated in terms of nociception produced by high throughput screening compounds thermal stimuli applied to the face of male Wistar rats, weighing 180-230 g. Electrical stimulation Amino acid transport inhibitor (bipolar and asymmetric square wave with 0.5 mA intensity for 20 min) of acupoint St36, located in the anterior tibial muscle 10 mm distal to the knee joint, induced antinociception in the present model, which was maintained for 150 min. Acupoint LI4, located in the junction of the first and second metacarpal

bones, did not achieve antinociception at any frequency studied (5 Hz: 1.7 +/- 0.1; 30 Hz: 1.8 +/- 0.1; 100 Hz: 1.7 +/- 0.1 vs 1.4 +/- 0.2). The antinociception obtained by stimulation of acupoint St36 was only achieved when high frequency 100 Hz (3.0 +/- 0.2 vs 1.0 +/- 0.1) was used, and not with 5 or 30 Hz (1.2 +/- 0.2 and 0.7 +/- 0.1 vs 1.0 +/- 0.1). The antinociceptive effect of acupuncture occurred by opioid pathway activation, since naloxone

(1 and 2 mg/kg, subcutaneously) antagonized it (1.8 +/- 0.2 and 1.7 +/- 0.2 vs 3.0 +/- 0.1).”
“Helicases are ubiquitous essential enzymes which have significant role in the nucleic acid metabolism. Using in silico approaches in the recent past we have identified a number of helicases in the Plasmodium falciparum genome. In the present study Belnacasan chemical structure we report purification and detailed characterization of a novel helicase from P. falciparum. Our results indicate that this helicase is a homologue of Dbp5 and DDX19 from yeast and human, respectively. The biochemical characterization shows that it contains DNA and RNA unwinding, nucleic acid dependent ATPase and RNA binding activities. It is interesting to note that this enzyme can unwind DNA duplexes in both 5′ to 3′ and 3′ to 5′ directions. Using truncated derivatives we further show that Q motif is essentially required for all of its activities. These studies should make an important contribution in understanding the enzymes involved in nucleic acid metabolism in the parasite. (C) 2010 Elsevier B.V. All rights reserved.”
“Axonal degeneration is a hallmark of many neurological disorders.