Parasitol Res 2004, 92:113–120.PubMedCrossRef 25. TriTryp DB: Kinetoplastid genomic resources Database. [http://triTrypdb.org/common/downloads/release-4.1/Tcruzi/fasta/TriTrypDB]
[] 26. Aslett M, Aurrecoechea C, Berriman M, Brestelli J, Brunk BP, Carrington M, Depledge DP, Fischer S, Gajria B, Gao X, Gardner MJ, Gingle A, Grant G, Harb OS, Heiges M, Hertz-Fowler C, Houston R, Innamorato F, Iodice J, Kissinger JC, Kraemer E, Li W, Logan FJ, Miller JA, Mitra S, Myler PJ, Nayak V, Pennington C, Phan I, Pinney DF: TriTrypDB: a functional genomic resource for the Trypanosomatidae. Nucleic Acids Res 2010, 38:457–462.CrossRef 27. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson WH-4-023 in vitro TJ, Higgins DG: Clustal W and Clustal X version 20. Bioinformatics 2007, 23:2947–2948.PubMedCrossRef 28. Gouy M, Guindon S, Gascuel O: SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree
building. Mol Biol Evol 2010, 27:221–224.PubMedCrossRef 29. Crooks GE, Hon G, Chandonia JM, Brenner SE: WebLogo: a sequence logo generator. Autophagy Compound Library Genome Res 2004, 14:1188–1190.PubMedCrossRef 30. Hirokawa T, Boon-Chieng S, Mitaku S: SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 1998, 14:378–379.PubMedCrossRef 31. Bendtsen JD, Nielsen H, von Heijne G, Brunak S: Improved prediction of signal peptides: SignalP 30. J Mol Biol 2004, 340:783–795.PubMedCrossRef 32. Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lages-Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm NR, Tibayrenc M, Schijman AG: A new consensus for this website Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem Inst Oswaldo Cruz 2009, 104:1051–1054.PubMedCrossRef
STK38 33. Cano MI, Gruber A, Vazquez M, Cortés A, Levin MJ, Gonzalez A, Degrave W, Rondinelli E, Ramirez JL, Alonso C, Requena JM, Franco Da Silveira J: Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi genome project. Mol Biochem Parasitol 1995, 7:273–278.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions MMK-M, LL and WDR carried out the molecular genetic studies, microscopy analyses, sequence alignments and phylogenetic analyses. RMCP and PRA participated in molecular genetic studies. RPM-Neto and DCB participated in the sequence and phylogenetic analyses. RAM participated in the microscopy analyses. WDR and SMRT designed and coordinated the study and drafted the manuscript. All authors have read and approved the final manuscript.