This NKT cell migration in vivo is arrested in liver sinusoids up

This NKT cell migration in vivo is arrested in liver sinusoids upon encounter with antigen presented on sinusoidal epithelial cells within minutes after injection of αGalCer.[64, ALK tumor 41, 65-67] In addition to antigen,

the IL-12 and IL-18 pro-inflammatory cytokines also terminate type I NKT cell motility in liver sinusoids of Cxcr6gfp/+ mice in a CD1d-independent manner. The latter arrest in NKT cell movement occurs by 1 hr after exposure to the cytokines and precedes NKT cell activation. Subsequent antigen encounter stabilizes the formation of an immune synapse between NKT cells and interacting APCs. This synapse elicits lymphocyte function-associated-1/intercellular adhesion molecule-1 interactions that enable activated type I NKT cells to be retained in the liver, demonstrating that activated type I NKT cells recirculate less than activated conventional CD4+ T cells.[68] However, after a stroke, type I NKT cells rapidly exit the liver and elicit bacteraemia. Similarly, NKT cells extravasate rapidly from the lung of αGalCer-treated mice and trigger inflammation and adaptive immune responses.[69] Hence, the patterns and kinetics of recirculation of type I mouse NKT cells differ in a tissue- and stimulus-dependent manner. Additional studies are required to unravel the mechanisms involved

and to determine whether this variation in recirculation exists for mouse type II NKT cells and human type I and type II NKT cells. Humans possess both CD4+ and CD4− type I NKT cells.[11] Although both subsets secrete Th1-type cytokines, find more CD4+ type I NKT cells secrete predominantly Th2-type cytokines. In a population of Th1-like CD4− NKT cells, CD8α+ cells comprise a large subset and CD8αβ+ cells a small subset. CD8α+ typeΙΝΚΤ cells secrete more IFN-γ and possess greater cytotoxic activity than do CD4+ or CD4− NKT cells. In human peripheral blood, type I NKT cells comprise about 0·1–0·2% of T cells, but this proportion is highly variable and can range

from < 0·1% to > 2%.[70-72] Twin studies suggest that the number of human type I NKT cells in PBMCs is genetically regulated.[4] Interestingly, human type I NKT cells are enriched in MycoClean Mycoplasma Removal Kit the omentum (about 10% of T cells) and not in the liver.[73, 74] Reduced numbers of type I NKT cells in PBMCs appear to correlate with several autoimmune or inflammatory conditions and cancers,[75] but this finding remains controversial. Similarly in patients with rheumatoid arthritis, PBMCs[76, 77] and synovia[78] display lower levels of NKT cells as well as a Th1 bias during disease.[77] Interestingly, patients with myasthenia gravis display elevated levels of type I NKT cells in PBMCs, in contrast to those in PBMCs from patients with MS,[75] rheumatoid arthritis[76] and type 1 diabetes[79]. The reason for these differences is currently unknown. Nevertheless, NKT cell levels return to normal levels after treatment.

Comments are closed.