1 0 6 76:1 28 2 30 9 15 6 Rice bran 47 9 2 2 12:1 35 5 26 3 5 4 M

1 0.6 76:1 28.2 30.9 15.6 Rice bran 47.9 2.2 12:1 35.5 26.3 5.4 Molasses 26.1 1.0 27:1 48.3 33.4 19.2 Leaves 16.2 4.5 45:1 – - – Grass clipping 30.3 3.6 15:1 28.6 24.5 – Mustard oil cake 39.4 1.8 26:1 40.6 19.6 33.5 Cow dung 24.8 1.5 20:1 37.2 21.6 20.4 Cow urine 11.6 16.3 0.8:1 – - – During the composting process, Rapamycin molecular weight the temperature

in the pile (5 to 30 cm from the top) was measured daily using a dry bulb thermometer. Similarly, the environment temperature was also recorded during composting near the pile. The samples were collected at every 10th day for microbial and physicochemical analysis. The composting was terminated after 50 days. The duplicate samples were used to assess the consistency or reproducibility in the method.

Physiochemical analysis of compost Compost pH and electrical conductivity (EC) were measured by preparing a (1:5 w v-1 compost: water) mixture as described by Rhoades [59] and Blakemore et al. [60] respectively. The percent organic carbon (C) in the compost was determined by the wet digestion method outlined by Walkley and Black [61]. Total nitrogen (N) was estimated by Kjeldahl method [62] and total sulfur according to the method of Steinbergs [63]. The potassium was selleck products estimated by ammonium-acetate method [64]. The samples were analyzed for micronutrient by atomic absorption spectrophotometer (Model 3030, Perkin-Elmer, USA). Macronutrients like calcium (Ca), magnesium (Mg) were determined following the methodology of Moral et al. [65] and sodium (Na) by using the method of Thompson and Wood [66]. The trace metals; CDK inhibitor copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) were estimated Anidulafungin (LY303366) by ICP-MS (Induced coupled plasma Mass Spectrometer) as per methodology of Koplık et al. [67]; Fingerová and Koplık [68]; Jenn-Hung and Shang-Lien [30], respectively. Isolation and enumeration of bacteria during composting Bacteria were isolated from compost by serial dilution method by plating 100 μl of diluted suspension from each phase the mesophile (30 and 35°C), thermophile (40 and 50°C), maturation and cooling phase (35 and 30°C) samples were spread plated on nutrient agar (NA) plates. The plates were incubated at 30°C,

35°C, 40°C and 50°C for 24 h. Colonies were counted and populations were expressed in term of cfu g-1. Morphologically different colonies were purified on NA plates. All isolates and were preserved on slants at 4°C and glycerol stock at -20°C in 20% (v v-1). All chemicals and media were of molecular grade and procured from either Merck Pvt. Ltd or Himedia, India. Morphological, biochemical and molecular characterization Presumptive identification was carried out by colony morphology and use of the first stage diagnostic biochemical tests for Gram-positive and Gram-negative bacteria. Further identification was carried out by standard biochemical tests by using Himedia tests kits (Hi motility™ and Assorted™ Biochemical kit, Hi Carbohydrate™ kit, Hi IMViC™ Biochemical test kit).

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