In addition, Eaf1 interacts with Vac8, which is required for several such aspects of vacuolar function. eaf3, eaf5, eaf7, eaf1 vid21 and yaf9 were identified as Opi mutants that result in overproduction of inositol, though not through direct repression of INO1. Mitchell et al. have observed SSL interactions of nonessential NuA4 components with proteins involved in membrane sorting protein traffick ing. They find that deletion the NuA4 component genes leads to defects in vacuolar morphology and similar to what we suggest for the phenotypic effects of tra1SRR3413, predict that the vacuolar defects arise from changes in gene expression. Mitochondrial function Fifteen genes annotated to have links to mitochondrial function were identified in the screen.

This may relate to the possible involvement of the SLIK complex in the ret rograde response pathway. Interestingly Inhibitors,Modulators,Libraries this pathway links mitochondrial metabolism with stress response, sig naling the expression of genes that provide required bio synthetic precursors during mitochondrial dysfunction, which results in the loss of tricarboxylic acid cycle activity. Stress response Included in the tra1SRR3413 SSL genes are greater than 30 related to cellular stress or stress response. Genetic interac tions Inhibitors,Modulators,Libraries for tra1SRR3413 with molecules related to cellular stress is consistent with the transcription profiles of strains containing deletions of SAGA SLIK and NuA4 compo nents as well as the calcofluor white, ethanol, rapamycin and temperature sensitivity of the tra1SRR3413 strain.

An aspect of the involvement of Tra1 in Inhibitors,Modulators,Libraries stress likely includes a role in the cell wall integrity Inhibitors,Modulators,Libraries response pathway Inhibitors,Modulators,Libraries as indicated by the calcofluor white sensitivity and inter actions of the tra1SRR3413 SSL profile with molecules required for membrane sorting protein trafficking and cell wall components. The useful handbook processes identified with Tra1 in many ways resemble those seen with Tor1, which as well as its initially defined role in integrating nutrient status with growth, has been genetically and functionally linked to membrane sorting protein trafficking, cytoskeletal events, microautophagy and to cell well integrity. The simplest interpretation of a possible link between Tra1 and stress is to suggest that Tra1 is involved in the expression of stress response genes or genes whose lack of expression results in stress. Consistent with this, in YPD media seventeen genes with an annotation or description indicative of an involvement in stress response have decreased expression of two fold or greater in a tra1SRR3413 background.